Immunogold Staining of Ultrathin Thawed Cryosections for Transmission Electron Microscopy (TEM)
نویسندگان
چکیده
منابع مشابه
Immunogold Staining of Ultrathin Thawed Cryosections for Transmission Electron Microscopy (TEM).
INTRODUCTIONA pre-embedding method of immunochemical staining is used if antigens are damaged by resin embedding, or if the best preservation of membranes is required. Applying immunogold reagents to sections of lightly fixed tissue, free of embedding medium, can be a very sensitive method of immunochemical staining. Cells or tissues are fixed as strongly as possible and then treated with a cry...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2008
ISSN: 1559-6095
DOI: 10.1101/pdb.prot5018